Analytical Scientist – Protein Characterisation (SEC-HPLC / SEC-MALS)

About the role

We design de novo cancer therapeutics computationally, the protein does not exist until we express and purify it. A design that looks perfect in silico may emerge as a clean monomer, a dimer, an aggregate, or several species that co-elute. Your job is to tell us what we actually made. SEC-HPLC will be your starting point.

What you'll do

• Develop, optimise and maintain SEC-HPLC methods for recombinant and de novo designed proteins for monomer purity, aggregate and fragment quantitation
• Establish SEC-MALS for absolute molecular weight, oligomeric state and aggregate characterisation
• Develop orthogonal methods: RP, IEX, HIC, and UHPLC/UPLC where resolution or run time demands it, using UV, PDA, ELSD and CAD detection as the molecule requires
• Adapt methods from academic and industry publications to our molecules, with the controls that show they transferred
• Write a clear analytical protocol for each method, and update it every time the method changes

What you need

• 2–5 yrs of experience
• HPLC
• LLMs
• Python
• R
• PhD

Good to have

• Chemistry
• GMP
• Git
• Documentation
• SOP writing